info@equi-genetique.comTél: 06.03.11.18.40
RReproduction, le choix logique : les résultats le prouvent

 

La congelation de sperme offre aux propriétaires d'étalons de multiples avantages :
- augmenter de manière significative le nombre de cartes vendues tous les ans par l'expédition sans limites géographiques de doses congelées.
- la possibilité pour un étalon de reproduire tout en assurant une carrière en compétition sans les contraintes de la reproduction.

-
la conservation illimitée dans le temps du patrimoine génétique d'un étalon.

Technique

Insémination artificielle classique
Le sperme, immédiatement après la récolte, est filtré puis dilué dans un 1er milieu. Un échantillon est analysé pour déterminer la qualité de l'éjaculât et le nombre total de spermatozoïdes ( si inférieur à 5 milliards, l'éjaculât est jeté). Lors de ces opérations, le sperme est maintenu à 37°.
Il est ensuite centrifugé entre 10 et 20mn afin de séparer les spermatozoïdes, plus denses, du plasma séminal.
Le surnageant est
éliminé et seul le culot de centrifugation est conservé (voir photo). Ce culot, qui contient tous les spermatozoïdes est remis en suspension avec un second milieu qui est élaboré pour permettre aux spermatozoïdes d'être congelés en subissant le moins de dommages possibles. Un nouvel échantillon est prélevé pour connaître le nombre de spermatozoïdes qui ont résisté à la centrifugation. Cette valeur sert à ajuster la concentration qui doit être de 100 millions de spermatozoïdes par millilitre. Lors de ces opérations, le sperme est maintenu à 22°.
Le sperme est ensuite descendu lentement en température jusqu'à 4°. Après 70 mn, le remplissage et le bouchage des paillettes est réalisé à 4° dans une banque réfrigérée. Les paillettes sont disposées sur un rack qui va être placé dans le bac de congélation. Pendant 10 mn, les paillettes sont maintenues à 4 cm au dessus de l'azote liquide(voir photo), à une température de -180°. Elles sont ensuite immergées dans l'azote liquide, à -196°.
Au moins 2 paillettes sont décongelées en les plongeant dans le bain-marie à 37°. La qualité du sperme est analysée pour déterminer si les paillettes sont conformes aux normes (entre 10 et 20% des éjaculas d'étalons parfaitement congelables sont jetés). Si la qualité est conforme, les paillettes sont rangées dans des visotubes identifiés qui sont sont eux même rangés dans les canisters d'une "bonbonne" remplie d'azote liquide.

Notre tarif congélation de sperme 2008 HT

Testage congélation (pension comprise)
Production de sperme congelé
Doses pour insémination classique
prix par dose : de 1 à 30 doses
de 31 à 75 doses
de 76 à 120 doses
au delà de 120 doses
Pension
Pension étalon (par jour)
Expédition de sperme congelé
Préparation des doses + azote liquide + envoi
aller-retour du container (en France métropolitaine)

Envois vers l'étranger (nous consulter)

700 €


39 €
36 €
33 €
30 €

12 €


150 €

En pratique
Le testage congélation, qui dure
entre 7 et 20 jours, sert à déterminer si le sperme d'un étalon est congelable. Après la purge, au moins 2 éjaculas sont congelés puis décongelés pour savoir si le sperme correspond au critère de qualité.
Une dose classique de sperme congelé : 8 paillettes de 0,5ml = 4ml de sperme concentré à 100 millions de spermatozoïdes par ml. 1 dose contient donc 400 millions de spermatozoïdes. Pour chaque insémination, il est utilisé 1 dose.
Une dose de sperme congelé "insémination profonde": 1 ou 2 paillettes de 0,5ml, soit 75 ou 150 millions de spermatozoïdes.

Toutes les paillettes sont identifiées : nom de l'étalon - sa race - son n° SIRE - la date de la récolte - le n° européen d'agrément du centre de production.
Une carte de saillie en congelé engage la fourniture de 4 doses par l'étalonnier . Les doses supplémentaires sont très souvent payantes.
Le critère de qualité de la semence congelée en France impose qu'il reste au moins 35% de spermatozoïdes vivants après la décongélation.

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